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Journal Articles Chemical Communications Year : 2013

High-throughput and long-term observation of compartmentalized biochemical oscillators

Abstract

We report the splitting of an oscillating DNA circuit into ∼ 700 droplets with picoliter volumes. Upon incubation at constant temperature, the droplets display sustained oscillations that can be observed for more than a day. Superimposed to the bulk behaviour, we find two intriguing new phenoma-slow desynchronization between the compartments and kinematic spatial waves-and investigate their possible origins. This approach provides a route to study the influence of small volume effects in biology, and paves the way to technological applications of compartmentalized molecular programs controlling complex dynamics.
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Dates and versions

hal-01901022 , version 1 (22-10-2018)

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Koshi Hasatani, Mathieu Leocmach, Anthony J Genot, André Estévez-Torres, Teruo Fujii, et al.. High-throughput and long-term observation of compartmentalized biochemical oscillators. Chemical Communications, 2013, 49 (73), ⟨10.1039/c3cc44323j⟩. ⟨hal-01901022⟩
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