What makes a good egg ? Molecular mechanisms defining egg developmental competence in teleost fish - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2015

What makes a good egg ? Molecular mechanisms defining egg developmental competence in teleost fish

Daniel Zarski
  • Fonction : Auteur
Iratxe Rojo
  • Fonction : Auteur

Résumé

Egg quality (i.e. the egg’s ability to be fertilized and subsequently develop into a normal embryo) is highly variable in the wild or under aquaculture conditions. Yet, the egg components – and associated molecular processes – responsible for its developmental competence remain poorly understood. In teleost fish, in which a high fecundity can be observed in comparison to other vertebrate models, it is possible to sample individual egg clutches in which both developmental success assessment and analytical studies can be performed in parallel. Several types of approaches have been conducted to draw the molecular portrait of a developmentally competent fish egg by studying its composition in terms of maternal mRNAs, miRNAs, and proteins. Correlative studies have shown a link between the abundance of specific mRNAs and/or proteins in the eggs and the overall developmental success of the corresponding egg clutches. In rainbow trout (Oncorhynchus mykiss), a correlation between developmental success and the abundance of nucleoplasmin (npm2) mRNA in the egg was previously established. In zebrafish (Danio rerio), a model species with transparent eggs and rapid development, a knockdown (KD) approach showed that maternally-inherited npm2 mRNA was crucial to allow developmental success beyond zygotic genome activation (ZGA). Similar approaches at the proteome and miRNA repertoire levels have yielded interesting results that are currently being further analyzed. Taking advantage of the wide diversity of fish models (over 30000 species), future studies will be designed to identify key molecular mechanisms that could be shared by evolutionary distant species.
Fichier principal
Vignette du fichier
2015_Bobe_GdRepro_1.pdf (5.52 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-01897087 , version 1 (03-06-2020)

Identifiants

  • HAL Id : hal-01897087 , version 1
  • PRODINRA : 369810

Citer

Julien Bobe, Aurélien Bouleau, Caroline Cheung, Ozlem Yilmaz, Thuy Thao Vi Nguyen, et al.. What makes a good egg ? Molecular mechanisms defining egg developmental competence in teleost fish. 1. Journées du GdR 3606 Repro, Université de Rennes 1 (UR1). Rennes, FRA., Apr 2015, Rennes, France. 105 p. ⟨hal-01897087⟩
37 Consultations
7 Téléchargements

Partager

Gmail Facebook X LinkedIn More