Porin self-association enables cell-to-cell contact in Providencia stuartii floating communities - Archive ouverte HAL
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2018

Porin self-association enables cell-to-cell contact in Providencia stuartii floating communities

Résumé

The gram-negative pathogen Providencia stuartii forms floating communities within which adjacent cells are in apparent contact, before depositing as canonical surface-attached biofilms. Because porins are the most abundant proteins in the outer membrane of gram-negative bacteria, we hypothesized that they could be involved in cell-to-cell contact and undertook a structure-function relationship study on the two porins of P. stuartii, Omp-Pst1 and Omp-Pst2. Our crystal structures reveal that these porins can self-associate through their extracellular loops, forming dimers of tri-mers (DOTs) that could enable cell-to-cell contact within floating communities. Support for this hypothesis was obtained by studying the porin-dependent aggregation of liposomes and model cells. The observation that facing channels are open in the two porin structures suggests that DOTs could not only promote cell-to-cell contact but also contribute to intercellular communication. biofilms | porins | intercellular communication | cell adhesion | steric zippers
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Dates et versions

hal-01894519 , version 1 (12-10-2018)

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Mariam El Khatib, Chady Nasrallah, Julie Lopes, Que-Tien Tran, Guillaume Tetreau, et al.. Porin self-association enables cell-to-cell contact in Providencia stuartii floating communities. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115 (10), pp.E2220-E2228. ⟨10.1073/pnas.1714582115⟩. ⟨hal-01894519⟩
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