Casein interaction with lipid membranes: Are the phase state or charge density of the phospholipids affecting protein adsorption? - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochimica et Biophysica Acta:Biomembranes Année : 2018

Casein interaction with lipid membranes: Are the phase state or charge density of the phospholipids affecting protein adsorption?

Franck Artzner
Cristelle Mériadec
Florence Rousseau
  • Fonction : Auteur
  • PersonId : 1203653
Christelle Lopez

Résumé

Casein micelles are ~200 nm electronegative particles that constitute 80 wt% of the milk proteins. During synthesis in the lactating mammary cells, caseins are thought to interact in the form of ~20 nm assemblies, directly with the biological membranes of the endoplasmic reticulum and/or the Golgi apparatus. However, conditions that drive this interaction are not yet known. Atomic force microscopy imaging and force spectroscopy were used to directly observe the adsorption of casein particles on supported phospholipid bilayers with controlled compositions to vary their phase state and surface charge density, as verified by X-ray diffraction and zetametry. At pH 6.7, the casein particles adsorbed onto bilayer phases with zwitterionic and liquid-disordered phospholipid molecules, but not on phases with anionic or ordered phospholipids. Furthermore, the presence of adsorbed caseins altered the stability of the yet exposed bilayer. Considering their respective compositions and symmetry/asymmetry, these results cast light on the possible interactions of casein assemblies with the organelles' membranes of the lactating mammary cells.
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Dates et versions

hal-01892948 , version 1 (09-11-2018)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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Adrian Crespo-Villanueva, Berta Gumi-Audenis, Fausto Sanz, Franck Artzner, Cristelle Mériadec, et al.. Casein interaction with lipid membranes: Are the phase state or charge density of the phospholipids affecting protein adsorption?. Biochimica et Biophysica Acta:Biomembranes, 2018, 1860 (12), pp.2588-2598. ⟨10.1016/j.bbamem.2018.09.016⟩. ⟨hal-01892948⟩
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