Functional and physical interaction between yeast Hsp90 and Hsp70 - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2018

Functional and physical interaction between yeast Hsp90 and Hsp70

Résumé

Heat shock protein 90 (Hsp90) is a highly conserved ATP-dependent molecular chaperone that is essential in eukaryotes. It is required for the activation and stabilization of more than 200 client proteins, including many kinases and steroid hormone receptors involved in cell-signaling pathways. Hsp90 chaperone activity requires collaboration with a subset of the many Hsp90 cochaperones, including the Hsp70 chaperone. In higher eukaryotes, the collaboration between Hsp90 and Hsp70 is indirect and involves Hop, a cochaperone that interacts with both Hsp90 and Hsp70. Here we show that yeast Hsp90 (Hsp82) and yeast Hsp70 (Ssa1), directly interact in vitro in the absence of the yeast Hop homolog (Sti1), and identify a region in the middle domain of yeast Hsp90 that is required for the interaction. In vivo results using Hsp90 substitution mutants showed that several residues in this region were important or essential for growth at high temperature. Moreover, mutants in this region were defective in interaction with Hsp70 in cell lysates. In vitro, the purified Hsp82 mutant proteins were defective in direct physical interaction with Ssa1 and in protein remodeling in collaboration with Ssa1 and cochaperones. This region of Hsp90 is also important for interactions with several Hsp90 cochaperones and client proteins , suggesting that collaboration between Hsp70 and Hsp90 in protein remodeling may be modulated through competition between Hsp70 and Hsp90 cochaperones for the interaction surface. Ssa1 | Hsp82 | molecular chaperones | Sti1 | Ydj1.
PNAS E2210.full.pdf (2.12 Mo) Télécharger le fichier

Dates et versions

hal-01890402 , version 1 (08-10-2018)

Licence

Paternité

Identifiants

Citer

Andrea Kravats, Joel R Hoskins, Michael Reidy, Jill L Johnson, Shannon Doyle, et al.. Functional and physical interaction between yeast Hsp90 and Hsp70. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115 (10), pp.E2210 - E2219. ⟨10.1073/pnas.1719969115⟩. ⟨hal-01890402⟩

Collections

CNRS UNIV-AMU
50 Consultations
68 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More