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Article Dans Une Revue Optics Letters Année : 2018

Dual-focus stimulated Raman scattering microscopy: a concept for multi-focus scaling

Dual-focus stimulated Raman scattering microscopy: a concept for multi-focus scaling

Sandro Heuke
  • Fonction : Auteur
Barbara Sarri
  • Fonction : Auteur
Alberto Lombardini
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  • PersonId : 989024
Xavier Audier
  • Fonction : Auteur
  • PersonId : 19219
  • IdHAL : xaudier
Herve Rigneault

Résumé

High speed imaging is of utmost importance for video-rate live cell investigations or to study extended sample areas at sufficient spatial resolution within reasonable time scales. Improving the speed of single-focus stimulated Raman scattering (SRS) microscopy is ultimately restricted by the sample's damage threshold and the shot noise of the demodulated laser source. To overcome this limitation we present a dual-focus SRS approach modulating the pump laser for each focus at a distinct frequency. The corresponding probe beams are detected each by a photo diode and demod-ulated individually by two separate lock-in units to avoid inter-focal cross-talk. Two laterally or axially displaced images as well as hyperspectral SRS images can be obtained simultaneously within the field of view of the objective lens. The modular implementation presented here can be extended to multiple foci by using multi-channel acousto-optics modulators in combination with multi-channel lock-in amplifiers.
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Dates et versions

hal-01885424 , version 1 (01-10-2018)

Identifiants

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Sandro Heuke, Barbara Sarri, Alberto Lombardini, Xavier Audier, Herve Rigneault. Dual-focus stimulated Raman scattering microscopy: a concept for multi-focus scaling. Optics Letters, 2018, 43 (19), ⟨10.1364/OL.43.004763⟩. ⟨hal-01885424⟩
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