Preventive effects of an original combination of grape seed polyphenols with amine fluoride on dental biofilm formation and oxidative damage by oral bacteria - Archive ouverte HAL Access content directly
Journal Articles Journal of Applied Microbiology Year : 2014

Preventive effects of an original combination of grape seed polyphenols with amine fluoride on dental biofilm formation and oxidative damage by oral bacteria

Christine Roques
  • Function : Author
  • PersonId : 1044666
C. Badet
  • Function : Author

Abstract

To investigate the preventive effects of an original combination of a grape seed extract (GSE) with an amine fluoride (Fluorinol(®) ) on dental plaque formation and oxidative damage caused by oral bacteria. METHODS AND RESULTS: The antibacterial activity of the compounds was assessed using the broth macrodilution method, and their antiplaque activity was evaluated on a multispecies biofilm grown on saliva-coated hydroxyapatite discs. The effect on glucosyltransferases activity was analysed through reductions in the overall reaction and the quantity of insoluble glucan synthesized. The combination of 2000 μg ml(-1) of GSE with 10·2 mg ml(-1) of Fluorinol(®) significantly decreased the biofilm formation (up to 4·76 log10 of reduction) and inhibited by 97·4% the insoluble glucan synthesis by glucosyltransferases. The antioxidant activity of this combination, alone or incorporated into a formulated mouthwash (Eludril daily(®) ), was determined using the Trolox equivalent antioxidant capacity assay (TEAC), and both showed significantly greater antioxidant capacity than vitamin C. CONCLUSIONS : The GSE/Fluorinol(®) combination showed both a significant antiplaque activity and an important antioxidant capacity in vitro, without any bactericidal effects. SIGNIFICANCE AND IMPACT OF THE STUDY: This is, to our knowledge, the first report on the properties of an original combination of a polyphenolic extract with amine fluoride that could be used for the prevention of oral diseases and oxidative damage associated.
Fichier principal
Vignette du fichier
Furiga_20321.pdf (181.7 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

hal-01878832 , version 1 (21-09-2018)

Identifiers

Cite

Aurélie Furiga, Christine Roques, C. Badet. Preventive effects of an original combination of grape seed polyphenols with amine fluoride on dental biofilm formation and oxidative damage by oral bacteria. Journal of Applied Microbiology, 2014, 116 (4), pp.761-771. ⟨10.1111/jam.12395⟩. ⟨hal-01878832⟩
25 View
120 Download

Altmetric

Share

Gmail Facebook X LinkedIn More