Statistical Optimization of Dilute Acid Hydrolysis of Wood Sawdust for Lactic Acid Production
Résumé
Sawdust wood (SW) is a promising lignocellulosic biomass for lactic acid production due to its abundant availability, low cost and high carbohydrates content that can be converted to fermentable sugars. The dilute acid hydrolysis of SW was investigated in this study and Lactococcus lactis was used to determine the feasibility of utilizing WS as feedstock for the production of lactic acid. Central composite design (CCD) was employed to optimize sulfuric acid, substrate loading and hydrolysis time for maximum sugar recovery. The results obtained were interpreted by analysis of variance and response surface methodology. The optimal acid hydrolysis conditions were as follows: sulfuric acid concentration of 8% (v/v) with substrate loading of 16% (w/v) and hydrolysis time of 2h. Under the optimal conditions, a glucose concentration of 8.78g/L was achieved. A regression model was generated according to the experimental data. Upon statistical analysis, coefficient of determination (R2) obtained was 0.96. Lactococcus lactis was found to be a potent lactic acid bacteria (LAB) strain for the production of lactic acid utilizing acid hydrolysate of WS and yeast extract as carbon and nitrogen sources, respectively. The maximum lactic acid concentration of 9.87g/L was obtained with a yield of 1.1g/g
Domaines
Sciences du Vivant [q-bio]
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Licence : CC BY NC - Paternité - Pas d'utilisation commerciale
Licence : CC BY NC - Paternité - Pas d'utilisation commerciale