Statistical Optimization of Dilute Acid Hydrolysis of Wood Sawdust for Lactic Acid Production - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Applied Biotechnology & Bioengineering Année : 2017

Statistical Optimization of Dilute Acid Hydrolysis of Wood Sawdust for Lactic Acid Production

Résumé

Sawdust wood (SW) is a promising lignocellulosic biomass for lactic acid production due to its abundant availability, low cost and high carbohydrates content that can be converted to fermentable sugars. The dilute acid hydrolysis of SW was investigated in this study and Lactococcus lactis was used to determine the feasibility of utilizing WS as feedstock for the production of lactic acid. Central composite design (CCD) was employed to optimize sulfuric acid, substrate loading and hydrolysis time for maximum sugar recovery. The results obtained were interpreted by analysis of variance and response surface methodology. The optimal acid hydrolysis conditions were as follows: sulfuric acid concentration of 8% (v/v) with substrate loading of 16% (w/v) and hydrolysis time of 2h. Under the optimal conditions, a glucose concentration of 8.78g/L was achieved. A regression model was generated according to the experimental data. Upon statistical analysis, coefficient of determination (R2) obtained was 0.96. Lactococcus lactis was found to be a potent lactic acid bacteria (LAB) strain for the production of lactic acid utilizing acid hydrolysate of WS and yeast extract as carbon and nitrogen sources, respectively. The maximum lactic acid concentration of 9.87g/L was obtained with a yield of 1.1g/g
Fichier principal
Vignette du fichier
JABB-04-00093.pdf (816.06 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Licence : CC BY NC - Paternité - Pas d'utilisation commerciale

Dates et versions

hal-01865707 , version 1 (21-04-2023)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Aicha Nancib, Naàssa Diboune, Nabil Nancib, Joseph Boudrant. Statistical Optimization of Dilute Acid Hydrolysis of Wood Sawdust for Lactic Acid Production. Journal of Applied Biotechnology & Bioengineering, 2017, 4 (1), ⟨10.15406/jabb.2017.04.00093⟩. ⟨hal-01865707⟩
45 Consultations
85 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More