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Article Dans Une Revue Biotechnology. Theory and practice. Année : 2013

Modulation of helicobacter pylori transcriptional profile by subinhibitory concentrations of rifampicin

Résumé

Subinhibitory concentrations (sub-MICs) of antibiotics do not kill bacteria, but they are able to interfere with important aspects of bacterial cell function, such as adhesion to host cells, surface bacterial energy, susceptibility to host defense mechanisms, inhibition of enzyme function and toxin production. In order to understand how H. pylori copes with environmental stress and what facilitates the emergence of RIF mutants in H. pylori, we used DNA microarrays to compare the gene expression profiles of H. pylori in the presence and absence of subinhibitory concentrations of rifampicin (1/16 MIC (0.1 mg/L), 1/8 MIC (0,2mg/L), ¼ MIC (0,4 mg/L), and ½ MIC (0,8 mg/L). We found that еhe expression of 57 genes (of the 1,576 genes analyzed) was increased more than ≥ 1,5-fold, and the expression of only 29 genes was decreased more than ≤ 1,5-fold in significant way (p-value < 0,05), when H. pylori was treated with sub-MICs of RIF. No correlation was found between the sub-MICs of RIF and gene expression. We conclude that the alteration in the transcriptional pattern of H. pylori after the exposure to sub-MICs of RIF is mainly due to a direct interaction between rifampicin and the RNA polymerase β-subunit. Finally, we propose that subinhibitory concentrations of rifampicin may lead to an increase in the number of hypermutable cells in the H. pylori population.
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Dates et versions

hal-01849414 , version 1 (05-06-2019)

Identifiants

Citer

K. Momynaliev, V. Chelysheva, O. Selezneva, A. Larin, T. Akopian, et al.. Modulation of helicobacter pylori transcriptional profile by subinhibitory concentrations of rifampicin. Biotechnology. Theory and practice., 2013, pp.23 - 29. ⟨10.11134/btp.2.2013.4⟩. ⟨hal-01849414⟩
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