Skeletal muscle expression of p43, a truncated thyroid hormone receptor α, affects lipid composition and metabolism - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Bioenergetics and Biomembranes Année : 2018

Skeletal muscle expression of p43, a truncated thyroid hormone receptor α, affects lipid composition and metabolism

Résumé

Thyroid hormone is a major regulator of metabolism and mitochondrial function. Thyroid hormone also affects reactions in almost all pathways of lipids metabolism and as such is considered as the main hormonal regulator of lipid biogenesis. The aim of this study was to explore the possible involvement of p43, a 43 Kda truncat ed form of the nuclear thyroid hormone receptor TR α1 which stimulates mitochondrial activity. Therefore, using mouse models overexpressing p43 in skeletal muscle (p43-Tg) or lacking p43 (p43−/−), we have investigated the lipid composition in quadriceps muscle and in mitochondria. Here, we reported in the quadriceps muscle of p43 −/−mice, a fall in triglycerides, an inhibition of monounsaturated fatty acids (MUFA) synthesis, an increase in elongase index and andecrease in desaturase index. However, in mitochondria from p43−/−mice, fatty acid profile was barely modified. In the quadriceps muscle of p43-Tg mice, MUFA content was decreased whereas the unsaturation index was increased. In addition, in quadriceps mitochondria of p43-Tg mice, we found an increase of linoleic acid level and unsaturation index. Last, we showed that cardiolipin content, a key phospholipid for mitochondrial function, remained unchanged both in quadriceps muscle and in its mitochondria whatever the mice genotype. In conclusion, this study shows that muscle lipid content and fatty acid profile are strongly affected in skeletal muscle by p43 levels. We also demonstrate that regulation of cardiolipin biosynthesis by the thyroid hormone does not imply p43.
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Dates et versions

hal-01837663 , version 1 (12-07-2018)

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François Casas, Gilles Fouret, Jérôme Lecomte, Fabienne Cortade, Laurence Pessemesse, et al.. Skeletal muscle expression of p43, a truncated thyroid hormone receptor α, affects lipid composition and metabolism. Journal of Bioenergetics and Biomembranes, 2018, 50 (1), pp.71-79. ⟨10.1007/s10863-018-9743-2⟩. ⟨hal-01837663⟩
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