Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa
Pauline Bernardo
(1, 2)
,
Brejnev Muhire
(3)
,
Sarah François
(4, 1, 5, 6, 7, 8, 9)
,
Maelle Deshoux
(1)
,
Penelope Hartnady
(3)
,
Kata Farkas
(10)
,
Simona Kraberger
(10)
,
Denis Filloux
(1, 2)
,
Emmanuel Fernandez
(1, 2)
,
Serge Galzi
(1, 2)
,
Romain Ferdinand
(1, 2)
,
Martine Granier
(1, 2)
,
Armelle Marais
(11)
,
Pablo Monge Blasco
(12)
,
Thierry T. Candresse
(11)
,
Fernando Escriu
(13)
,
Arvind Varsani
(10, 14, 15)
,
Gordon W. Harkins
(16)
,
Darren P. Martin
(3)
,
Philippe Roumagnac
(1, 2)
1
UMR BGPI -
Biologie et Génétique des Interactions Plante-Parasite
2 Cirad - Centre de Coopération Internationale en Recherche Agronomique pour le Développement
3 Computational Biology Group, Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences
4 DGIMI - Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier]
5 INRA - Institut National de la Recherche Agronomique
6 Montpellier SupAgro - Institut national d’études supérieures agronomiques de Montpellier
7 MIVEGEC - Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle
8 CNRS - Centre National de la Recherche Scientifique
9 IRD - Institut de Recherche pour le Développement
10 School of Biological Sciences and Biomolecular Interaction Centre
11 BFP - Biologie du fruit et pathologie
12 Unidad de Sanidad Vegetal
13 Unidad de Sanidad Vegetal
14 Department of Plant Pathology and Emerging Pathogens Institute
15 Structural Biology Research Unit, Department of Clinical Laboratory Sciences
16 South African National Bioinformatics Institute, MRC Unit for Bioinformatics Capacity Development
2 Cirad - Centre de Coopération Internationale en Recherche Agronomique pour le Développement
3 Computational Biology Group, Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences
4 DGIMI - Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier]
5 INRA - Institut National de la Recherche Agronomique
6 Montpellier SupAgro - Institut national d’études supérieures agronomiques de Montpellier
7 MIVEGEC - Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle
8 CNRS - Centre National de la Recherche Scientifique
9 IRD - Institut de Recherche pour le Développement
10 School of Biological Sciences and Biomolecular Interaction Centre
11 BFP - Biologie du fruit et pathologie
12 Unidad de Sanidad Vegetal
13 Unidad de Sanidad Vegetal
14 Department of Plant Pathology and Emerging Pathogens Institute
15 Structural Biology Research Unit, Department of Clinical Laboratory Sciences
16 South African National Bioinformatics Institute, MRC Unit for Bioinformatics Capacity Development
Sarah François
- Fonction : Auteur
- PersonId : 1321413
- IdHAL : sarah-francois
- ORCID : 0000-0002-1138-8800
Simona Kraberger
- Fonction : Auteur
- PersonId : 780666
- ORCID : 0000-0002-7037-9242
Armelle Marais
- Fonction : Auteur
- PersonId : 740106
- IdHAL : armelle-marais
- ORCID : 0000-0003-2482-1543
- IdRef : 25311957X
Thierry T. Candresse
- Fonction : Auteur
- PersonId : 741063
- IdHAL : thierry-candresse
- ORCID : 0000-0001-9757-1835
- IdRef : 058616438
Philippe Roumagnac
- Fonction : Auteur
- PersonId : 1137455
Résumé
Little is known about the prevalence, diversity, evolutionary processes, genomic structures and population dynamics of viruses in the divergent geminivirus lineage known as the capulaviruses. We determined and analyzed full genome sequences of 13 Euphorbia caput-medusae latent virus (EcmLV) and 26 Alfalfa leaf curl virus (ALCV) isolates, and partial genome sequences of 23 EcmLV and 37 ALCV isolates. While EcmLV was asymptomatic in uncultivated southern African Euphorbia caput-medusae, severe alfalfa disease symptoms were associated with ALCV in southern France. The prevalence of both viruses exceeded 10% in their respective hosts. Besides using patterns of detectable negative selection to identify ORFs that are probably functionally expressed, we show that ALCV and EcmLV both display evidence of inter-species recombination and biologically functional genomic secondary structures. Finally, we show that whereas the EcmLV populations likely experience restricted geographical dispersion, ALCV is probably freely moving across the French Mediterranean region.
Format du dépôt | Notice |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa
|
Résumé |
en
Little is known about the prevalence, diversity, evolutionary processes, genomic structures and population dynamics of viruses in the divergent geminivirus lineage known as the capulaviruses. We determined and analyzed full genome sequences of 13 Euphorbia caput-medusae latent virus (EcmLV) and 26 Alfalfa leaf curl virus (ALCV) isolates, and partial genome sequences of 23 EcmLV and 37 ALCV isolates. While EcmLV was asymptomatic in uncultivated southern African Euphorbia caput-medusae, severe alfalfa disease symptoms were associated with ALCV in southern France. The prevalence of both viruses exceeded 10% in their respective hosts. Besides using patterns of detectable negative selection to identify ORFs that are probably functionally expressed, we show that ALCV and EcmLV both display evidence of inter-species recombination and biologically functional genomic secondary structures. Finally, we show that whereas the EcmLV populations likely experience restricted geographical dispersion, ALCV is probably freely moving across the French Mediterranean region.
|
Auteur(s) |
Pauline Bernardo
1, 2
, Brejnev Muhire
3
, Sarah François
4, 1, 5, 6, 7, 8, 9
, Maelle Deshoux
1
, Penelope Hartnady
3
, Kata Farkas
10
, Simona Kraberger
10
, Denis Filloux
1, 2
, Emmanuel Fernandez
1, 2
, Serge Galzi
1, 2
, Romain Ferdinand
1, 2
, Martine Granier
1, 2
, Armelle Marais
11
, Pablo Monge Blasco
12
, Thierry T. Candresse
11
, Fernando Escriu
13
, Arvind Varsani
10, 14, 15
, Gordon W. Harkins
16
, Darren P. Martin
3
, Philippe Roumagnac
1, 2
1
UMR BGPI -
Biologie et Génétique des Interactions Plante-Parasite
( 73810 )
- CIRAD
UMR-BGPI TA A-54/K - Campus International de Baillarguet - 34398 Montpellier Cedex 5
- France
2
Cirad -
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
( 11574 )
- Siège 42, rue Scheffer 75116 Paris
- France
3
Computational Biology Group, Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences
( 493076 )
- Afrique du Sud
4
DGIMI -
Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier]
( 189647 )
- Place Eugène Bataillon 34095 Montpellier cedex 5
- France
5
INRA -
Institut National de la Recherche Agronomique
( 92114 )
- France
6
Montpellier SupAgro -
Institut national d’études supérieures agronomiques de Montpellier
( 474617 )
- 2, place Viala
34060 Montpellier Cédex 2
- France
7
MIVEGEC -
Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle
( 166573 )
- 911 Avenue Agropolis BP 64501 34394 Montpellier cedex 5
- France
8
CNRS -
Centre National de la Recherche Scientifique
( 441569 )
- France
9
IRD -
Institut de Recherche pour le Développement
( 67872 )
- Siège
Le Sextant
44, bd de Dunkerque
CS 90009
13572 Marseille cedex 02
- France
10
School of Biological Sciences and Biomolecular Interaction Centre
( 514222 )
- Nouvelle-Zélande
11
BFP -
Biologie du fruit et pathologie
( 208863 )
- Centre INRA Bordeaux-Aquitaine 71 avenue Bourlaux BP81 F-33883 Villenave d'Ornon
- France
12
Unidad de Sanidad Vegetal
( 538671 )
- Espagne
13
Unidad de Sanidad Vegetal
( 538672 )
- Espagne
14
Department of Plant Pathology and Emerging Pathogens Institute
( 538673 )
- États-Unis
15
Structural Biology Research Unit, Department of Clinical Laboratory Sciences
( 514224 )
- Afrique du Sud
16
South African National Bioinformatics Institute, MRC Unit for Bioinformatics Capacity Development
( 538674 )
- Afrique du Sud
|
Localisation géographique du document |
Erist, Montpellier (Publis16-bgpi-007)
|
Public visé |
Scientifique
|
Sous-type de document pour les Articles |
Research article
|
Version du document |
version éditeur
|
Langue du document |
Anglais
|
Nom de la revue |
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Non spécifiée
|
Date de publication |
2016
|
Volume |
493
|
Page/Identifiant |
142-153
|
Commentaire |
BGPI : équipe 6/7
|
Voir aussi |
|
Domaine(s) |
|
Financement |
|
Indexation contrôlée |
|
Mots-clés |
en
geminiviridae, alfalfa leaf curl virus, euphorbia caput-medusae latent virus, prevalence, recombination, secondary structure, genome organization, France, Spain, South Africa
fr
maladie des plantes, pathologie végétale, maladie virale, afrique du sud
|
DOI | 10.1016/j.virol.2016.03.016 |
ProdINRA | 351207 |
UT key WOS | 000375824300014 |
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