Beef tenderness and intramuscular fat proteomic biomarkers: muscle type effect
Résumé
Tenderness and intramuscular fat content are key attributes for beef sensory qualities.
Recently some proteomic analysis revealed several proteins which are considered as
good biomarkers of these quality traits. This study focuses on the analysis of 20 of
these proteins representative of several biological functions: muscle structure and
ultrastructure, muscle energetic metabolism, cellular stress and apoptosis. The relative
abundance of the proteins was measured by Reverse Phase Protein Array (RPPA) in
five muscles known to have different tenderness and intramuscular lipid contents:
Longissimus thoracis (LT), Semimembranosus (SM), Rectus abdominis (RA), Triceps
brachii (TB) and Semitendinosus (ST). The main results showed a muscle type effect
on 16 among the 20 analyzed proteins. They revealed differences in protein abundance
depending on the contractile and metabolic properties of the muscles. The RA muscle
was the most different by 11 proteins differentially abundant comparatively to the
four other muscles. Among these 11 proteins, six were less abundant namely enolase
3 (ENO3), phosphoglucomutase 1 (PGK1), aldolase (ALDOA), myosin heavy chain
IIX (MyHC-IIX), fast myosin light chain 1 (MLC1F), triosephosphate isomerase 1
(TPI1) and five more abundant: Heat shock protein (HSP27, HSP70-1A1, B-crystallin
(CRYAB), troponin T slow (TNNT1), and aldolase dehydrogenase 1 (ALDH1A1).
Four proteins: HSP40, four and a half LIM domains protein 1 (FHL1), glycogen
phosphorylase B (PYGB) and malate dehydrogenase (MDH1) showed the same
abundance whatever the muscle. The correlations observed between the 20 proteins
in all the five muscles were used to construct a correlation network. The proteins the
most connected with the others were in the following order MyHC-IIX, CRYAB, TPI1,
PGK1, ALDH1A1, HSP27 and TNNT1. This knowledge is important for understanding
the biological functions related to beef tenderness and intramuscular fat content.
Origine | Accord explicite pour ce dépôt |
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