Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Methods in Molecular Biology Année : 2018

Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy.

Résumé

In this chapter, we describe how NMR chemical shift titrations can be used to study the interaction between two proteins with emphasis on mapping the interface of the complex and determining the binding affinity from a quantitative analysis of the experimental data. In particular, we discuss the appearance of NMR spectra in different chemical exchange regimes (fast, intermediate, and slow) and how these regimes affect NMR data analysis.
Fichier non déposé

Dates et versions

hal-01807808 , version 1 (05-06-2018)

Identifiants

Citer

Jose Luis Ortega-Roldan, Martin Blackledge, Malene Ringkjøbing Jensen. Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy.. Methods in Molecular Biology, 2018, 1764, pp.73-85. ⟨10.1007/978-1-4939-7759-8_5⟩. ⟨hal-01807808⟩
59 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More