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Journal Articles Methods in Molecular Biology Year : 2018

Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy.

Abstract

In this chapter, we describe how NMR chemical shift titrations can be used to study the interaction between two proteins with emphasis on mapping the interface of the complex and determining the binding affinity from a quantitative analysis of the experimental data. In particular, we discuss the appearance of NMR spectra in different chemical exchange regimes (fast, intermediate, and slow) and how these regimes affect NMR data analysis.
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hal-01807808 , version 1 (05-06-2018)

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Jose Luis Ortega-Roldan, Martin Blackledge, Malene Ringkjøbing Jensen. Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy.. Methods in Molecular Biology, 2018, 1764, pp.73-85. ⟨10.1007/978-1-4939-7759-8_5⟩. ⟨hal-01807808⟩
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