Article Dans Une Revue Methods in Molecular Biology Année : 2016

Histone purification from Saccharomyces cerevisiae

Résumé

The nucleosome structure consists of a histone octamer made by a tetramer of H3-­‐H4 histones and two dimers of H2A-­‐H2B. Nucleosomes undergo extensive post-­‐ translational modifications that regulate nucleosome interactions, position, and stability. We describe a protocol allowing the robust purification of histones from the yeast Saccharomyces cerevisiae. This method appears to be suitable to quantitatively analyse specific posttranslational histone modifications.

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hal-01796569 , version 1 (21-05-2018)

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Frederic Jourquin, Vincent Géli. Histone purification from Saccharomyces cerevisiae. Methods in Molecular Biology, 2016, Histones, 1528, pp.69-73. ⟨10.1007/978-1-4939-6630-1_5⟩. ⟨hal-01796569⟩

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