The nucleosome structure consists of a histone octamer made by a tetramer of H3-­‐H4 histones and two dimers of H2A-­‐H2B. Nucleosomes undergo extensive post-­‐ translational modifications that regulate nucleosome interactions, position, and stability. We describe a protocol allowing the robust purification of histones from the yeast Saccharomyces cerevisiae. This method appears to be suitable to quantitatively analyse specific posttranslational histone modifications.