An NF90/NF110-mediated feedback amplification loop regulates dicer expression and controls ovarian carcinoma progression - Archive ouverte HAL
Article Dans Une Revue Cell Research Année : 2018

An NF90/NF110-mediated feedback amplification loop regulates dicer expression and controls ovarian carcinoma progression

Xavier Contreras
Lisa Bluy
  • Fonction : Auteur
  • PersonId : 744582
  • IdHAL : lisa-bluy
Sylvie Rouquier
William Ritchie
Rosemary Kiernan

Résumé

Reduced expression of DICER, a key enzyme in the miRNA pathway, is frequently associated with aggressive, invasive disease, and poor survival in various malignancies. Regulation of DICER expression is, however, poorly understood. Here, we show that NF90/NF110 facilitates DICER expression by controlling the processing of a miRNA, miR-3173, which is embedded in DICER pre-mRNA. As miR-3173 in turn targets NF90, a feedback amplification loop controlling DICER expression is established. In a nude mouse model, NF90 overexpression reduced proliferation of ovarian cancer cells and significantly reduced tumor size and metastasis, whereas overexpression of miR-3173 dramatically increased metastasis in an NF90- and DICER-dependent manner. Clinically, low NF90 expression and high miR-3173-3p expression were found to be independent prognostic markers of poor survival in a cohort of ovarian carcinoma patients. These findings suggest that, by facilitating DICER expression, NF90 can act as a suppressor of ovarian carcinoma.

Dates et versions

hal-01794993 , version 1 (18-05-2018)

Identifiants

Citer

Jérome Barbier, Xin Chen, Gabriel Sanchez, Muyan Cai, Marion Helsmoortel, et al.. An NF90/NF110-mediated feedback amplification loop regulates dicer expression and controls ovarian carcinoma progression. Cell Research, 2018, 28 (5), pp.556 - 571. ⟨10.1038/s41422-018-0016-8⟩. ⟨hal-01794993⟩
50 Consultations
0 Téléchargements

Altmetric

Partager

More