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Journal Articles Biophysical Journal Year : 2017

Interfacial Properties of N TAIL , an Intrinsically Disordered Protein

Abstract

Intrinsically disordered proteins (IDPs) lack stable secondary and tertiary structure under physiological conditions in the absence of their biological partners and thus exist as dynamic ensembles of interconverting conformers, often highly soluble in water. However, in some cases, IDPs such as the ones involved in neurodegenerative diseases can form protein aggregates and their aggregation process may be triggered by the interaction with membranes. Although the interfacial behavior of globular proteins has been extensively studied, experimental data on IDPs at the air/water (A/W) and water/lipid interfaces are scarce. We studied here the intrinsically disordered C-terminal domain of the Hendra virus nucleoprotein (N TAIL) and compared its interfacial properties to those of lysozyme that is taken as a model globular protein of similar molecular mass. Adsorption of N TAIL at the A/W interface was studied in the absence and presence of phospholipids using Langmuir films, polarization modulated-infrared reflection-absorption spectroscopy, and an automated drop tensiometer for interfacial tension and elastic modulus determination with oscillating bubbles. N TAIL showed a significant surface activity, with a higher adsorption capacity at the A/W interface and penetration into egg phosphatidylcholine monolayer compared to lysozyme. Whereas lysozyme remains folded upon compression of the protein layer at the A/W interface and shows a quasi-pure elastic behavior, N TAIL shows a much higher molecular area and forms a highly visco-elastic film with a high dilational modulus. To our knowledge, a new disorder-to-order transition is thus observed for the N TAIL protein that folds into an antiparallel b-sheet at the A/W interface and presents strong intermolecular interactions.

Domains

Biophysics
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Dates and versions

hal-01791747 , version 1 (18-05-2018)

Identifiers

Cite

Anaïs Benarouche, Johnny Habchi, Alain Cagna, Ofelia Maniti, Agnès P Girard-Egrot, et al.. Interfacial Properties of N TAIL , an Intrinsically Disordered Protein. Biophysical Journal, 2017, 113 (12), pp.2723-2735. ⟨10.1016/j.bpj.2017.10.010⟩. ⟨hal-01791747⟩
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