Regulation of NUB1 Activity through Non- Proteolytic Mdm2-Mediated Ubiquitination - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2017

Regulation of NUB1 Activity through Non- Proteolytic Mdm2-Mediated Ubiquitination

Résumé

NUB1 (Nedd8 ultimate buster 1) is an adaptor protein which negatively regulates the ubiqui-tin-like protein Nedd8 as well as neddylated proteins levels through proteasomal degradation. However, molecular mechanisms underlying this function are not completely understood. Here, we report that the oncogenic E3 ubiquitin ligase Mdm2 is a new NUB1 interacting protein which induces its ubiquitination. Interestingly, we found that Mdm2-mediated ubiquitination of NUB1 is not a proteolytic signal. Instead of promoting the conjugation of polyubiquitin chains and the subsequent proteasomal degradation of NUB1, Mdm2 rather induces its di-ubiquitination on lysine 159. Importantly, mutation of lysine 159 into arginine inhibits NUB1 activity by impairing its negative regulation of Nedd8 and of neddylated proteins. We conclude that Mdm2 acts as a positive regulator of NUB1 function, by modulating NUB1 ubiquitination on lysine 159.
Fichier principal
Vignette du fichier
Bonacci_PlosOne_2017.pdf (3.08 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01787875 , version 1 (07-05-2018)

Identifiants

  • HAL Id : hal-01787875 , version 1

Citer

Thomas Bonacci, Stéphane Audebert, Luc Camoin, Emilie Baudelet, Juan-Lucio Iovanna, et al.. Regulation of NUB1 Activity through Non- Proteolytic Mdm2-Mediated Ubiquitination. PLoS ONE, 2017. ⟨hal-01787875⟩
41 Consultations
36 Téléchargements

Partager

Gmail Facebook X LinkedIn More