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Article Dans Une Revue Current Microbiology Année : 2018

Characterization of a New Ezakiella Isolated from the Human Vagina: Genome Sequence and Description of Ezakiella massiliensis sp nov.

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The study of the vaginal microbiota using the ``culturomics concept'' allowed us to isolate, from the vaginal swab of an asymptomatic 20-year-old woman who had sexual relations with another woman with bacterial vaginosis, an unknown Gram-positive anaerobic coccus-shaped bacterium that was designated strain Marseille-P2951(T) and characterized using taxono-genomics. Strain Marseille-P2951(T) is non-motile and non-spore forming and exhibits catalase and oxidase activities. Its 16S rRNA gene-based identification showed 98.5% identity with Ezakiella peruensis, the phylogenetically closest species. The major fatty acids are C18:1n9 (58%) and C16:0 (22%). With a 1,741,785 bp length, the G+C content of the genome is 36.69%. Of a total of 1657 genes, 1606 are protein-coding genes and 51 RNAs. Also, 1123 genes are assigned a putative function and 127 are ORFans. Phenotypic, phylogenetic, and genomics analyses revealed that strain Marseille-P2951(T) (=CSUR P2951 =DSM 103122) is distinct and represents a new species of the genus Ezakiella, for which the name Ezakiella massiliensis sp. nov. is proposed.
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hal-01780663 , version 1 (17-09-2021)

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Khoudia Diop, Claudia Andrieu, Caroline Michelle, Nicholas Armstrong, Fadi Bittar, et al.. Characterization of a New Ezakiella Isolated from the Human Vagina: Genome Sequence and Description of Ezakiella massiliensis sp nov.. Current Microbiology, 2018, 75 (4), pp.456-463. ⟨10.1007/s00284-017-1402-z⟩. ⟨hal-01780663⟩
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