Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms - Archive ouverte HAL
Article Dans Une Revue Nature Methods Année : 2013

Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms

Résumé

We established a conditional site–specific recombination system based on dimerizable Cre– mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a novel single vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicomplexan genes considered essential for host-cell invasion. Our findings uncover the existence of an alternative invasion pathway in apicomplexan parasites.

Domaines

Parasitologie
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Dates et versions

hal-01772103 , version 1 (20-04-2018)

Identifiants

Citer

Nicole Andenmatten, Saskia Egarter, Allison J Jackson, Nicolas Jullien, Jean-Paul Herman, et al.. Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms. Nature Methods, 2013, 10 (2), pp.125-127. ⟨10.1038/nmeth.2301⟩. ⟨hal-01772103⟩
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