Physico-Chemical Characterization of the Interaction of Red Fluorescent Protein—DsRed With Thin Silica Layers
Résumé
The Discosoma recombinant red fluorescent (DsRed) protein is the latest member of the family of fluorescent proteins. It holds great promise for applications in biotechnology and cell biology. However, before being used for rational engineering, knowledge on the behavior of DsRed and the underlying mechanisms relating its structural stability and adsorption properties on solid surfaces is highly demanded. The physico-chemical analysis performed in this study reveals that the interaction of DsRed with SiO2 surfaces does not lead to complete protein denaturation after adsorption and dehydration. Nevertheless, the photoluminescence emission of dehydrated DsRed small droplets was found to be slightly red-shifted, peaking at 590 nm. The measured contact angles of droplets containing different concentration of DsRed proteins determine the interaction as hydrophilic one, however with larger contact angles for larger DsRed concentrations. The DsRed protein behavior is not pH-dependent with respect of the contact angle measurements, in agreement with previously reported studies.
Mots clés
Red fluorescent proteins
Physico-chemical characterization
Physico-chemical analysis
Photoluminescence emission
pH
Hydrogen-Ion Concentration
red fluorescent protein
chemistry
Recombinant proteins
photoprotein
Chemical analysis
Contact angle
Cytology
Drop formation
Drops
Fluorescence
Stability
Adsorption properties
Luminescent Proteins
infrared spectroscopy
Silicon Dioxide
Materials Testing
Water
Fourier Transform Infrared
Spectroscopy
DsRed
Structural stabilities
thin SiO<sub>2</sub> layers
Proteins
Thin SiO2 layers
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