Article Dans Une Revue Molecular and Cellular Biology Année : 2007

P-Body Formation Is a Consequence, Not the Cause, of RNA-Mediated Gene Silencing

Résumé

P bodies are cytoplasmic domains that contain proteins involved in diverse posttranscriptional processes, such as mRNA degradation, nonsense-mediated mRNA decay (NMD), translational repression, and RNA-mediated gene silencing. The localization of these proteins and their targets in P bodies raises the question of whether their spatial concentration in discrete cytoplasmic domains is required for posttranscriptional gene regulation. We show that processes such as mRNA decay, NMD, and RNA-mediated gene silencing are functional in cells lacking detectable microscopic P bodies. Although P bodies are not required for silencing, blocking small interfering RNA or microRNA silencing pathways at any step prevents P-body formation, indicating that P bodies arise as a consequence of silencing. Consistently, we show that releasing mRNAs from polysomes is insufficient to trigger P-body assembly: polysome-free mRNAs must enter silencing and/or decapping pathways to nucleate P bodies. Thus, even though P-body components play crucial roles in mRNA silencing and decay, aggregation into P bodies is not required for function but is instead a consequence of their activity.

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hal-01738446 , version 1 (20-03-2018)

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Ana Eulalio, Isabelle Behm-Ansmant, Daniel Schweizer, Elisa Izaurralde. P-Body Formation Is a Consequence, Not the Cause, of RNA-Mediated Gene Silencing. Molecular and Cellular Biology, 2007, 27 (11), pp.3970-3981. ⟨10.1128/MCB.00128-07⟩. ⟨hal-01738446⟩
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