Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA - Archive ouverte HAL
Article Dans Une Revue Journal of Nucleic Acids Année : 2011

Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA

Résumé

Naturally occurring cellular RNAs contain an impressive number of chemically distinct modified residues which appear posttranscriptionally, as a result of specific action of the corresponding RNA modification enzymes. Over 100 different chemical modifications have been identified and characterized up to now. Identification of the chemical nature and exact position of these modifications is typically based on 2D-TLC analysis of nucleotide digests, on HPLC coupled with mass spectrometry, or on the use of primer extension by reverse transcriptase. However, many modified nucleotides are silent in reverse transcription, since the presence of additional chemical groups frequently does not change base-pairing properties. In this paper, we give a summary of various chemical approaches exploiting the specific reactivity of modified nucleotides in RNA for their detection.
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Dates et versions

hal-01738372 , version 1 (20-03-2018)

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Isabelle Behm-Ansmant, Mark Helm, Yuri Motorin. Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA. Journal of Nucleic Acids , 2011, 2011, pp.1 - 17. ⟨10.4061/2011/408053⟩. ⟨hal-01738372⟩
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