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Article Dans Une Revue Cell Stem Cell Année : 2013

Reprogramming of human fibroblasts to pluripotency with lineage specifiers

Nuria Montserrat
  • Fonction : Auteur
Ignacio Sancho-Martinez
  • Fonction : Auteur
Tomoaki Hishida
  • Fonction : Auteur
Laia Miquel
  • Fonction : Auteur
Carme Cortina
  • Fonction : Auteur
Yuriko Hishida
  • Fonction : Auteur
Concepcion Rodriguez Esteban
  • Fonction : Auteur
Juan Carlos Izpisua Belmonte
  • Fonction : Auteur

Résumé

Since the initial discovery that OCT4, SOX2, KLF4, and c-MYC overexpression sufficed for the induction of pluripotency in somatic cells, methodologies replacing the original factors have enhanced our understanding of the reprogramming process. However, unlike in mouse, OCT4 has not been replaced successfully during reprogramming of human cells. Here we report on a strategy to accomplish this replacement. Through a combination of transcriptome and bioinformatic analysis we have identified factors previously characterized as being lineage specifiers that are able to replace OCT4 and SOX2 in the reprogramming of human fibroblasts. Our results show that it is possible to replace OCT4 and SOX2 simultaneously with alternative lineage specifiers in the reprogramming of human cells. At a broader level, they also support a model in which counteracting lineage specification networks underlies the induction of pluripotency.

Dates et versions

hal-01736201 , version 1 (16-03-2018)

Identifiants

Citer

Nuria Montserrat, Emmanuel Nivet, Ignacio Sancho-Martinez, Tomoaki Hishida, Sachin Kumar, et al.. Reprogramming of human fibroblasts to pluripotency with lineage specifiers. Cell Stem Cell, 2013, 13 (3), pp.341--350. ⟨10.1016/j.stem.2013.06.019⟩. ⟨hal-01736201⟩
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