In this work, we introduce a novel simulation game platform to study the route choice behaviour of travellers. A unique feature of this game is that it interacts with a microscopic simulator, which is in charge of reproducing the traffic conditions over real road networks, such as Lyon-Villeurbanne (36km2 and 3,222 nodes). Multiple OD pairs can be assigned to players during one game session. The participants play trips changing their departure times and routes, and thus modifying the original assignment of the simulation in real-time. Thus, the simulation game permits to investigate not only the determinants of player decisions (travel time, characteristics of the alternatives, traffic information), but also how concomitant decisions from multiple players impact the network.