Control of MT1-MMP transport by atypical PKC during breast-cancer progression - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2014

Control of MT1-MMP transport by atypical PKC during breast-cancer progression

Catalina Lodillinsky
Laetitia Fuhrmann
Maya Nourieh
  • Fonction : Auteur
Emilie Lagoutte
  • Fonction : Auteur
Mark Linch
  • Fonction : Auteur
Johan van Lint
  • Fonction : Auteur
Graça Raposo
Anne Vincent-Salomon
Peter Parker
  • Fonction : Auteur
C. Rosse
  • Fonction : Auteur

Résumé

Dissemination of carcinoma cells requires the pericellular degradation of the extracellular matrix, which is mediated by membrane type 1-matrix metalloproteinase (MT1-MMP). In this article, we report a co-up-regulation and colocalization of MT1-MMP and atypical protein kinase C iota (aPKCι) in hormone receptor-negative breast tumors in association with a higher risk of metastasis. Silencing of aPKC in invasive breast-tumor cell lines impaired the delivery of MT1-MMP from late endocytic storage compartments to the surface and inhibited matrix degradation and invasion. We provide evidence that aPKCι, in association with MT1-MMP-containing endosomes, phosphorylates cortactin, which is present in F-actin-rich puncta on MT1-MMP-positive endosomes and regulates cortactin association with the membrane scission protein dynamin-2. Thus, cell line-based observations and clinical data reveal the concerted activity of aPKC, cortactin, and dynamin-2, which control the trafficking of MT1-MMP from late endosome to the plasma membrane and play an important role in the invasive potential of breast-cancer cells.
Fichier principal
Vignette du fichier
CONICET_Digital_Nro.20763.pdf (1.44 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-01712228 , version 1 (19-12-2023)

Identifiants

Citer

Carine Rossé, Catalina Lodillinsky, Laetitia Fuhrmann, Maya Nourieh, Pedro Monteiro, et al.. Control of MT1-MMP transport by atypical PKC during breast-cancer progression. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111 (18), pp.E1872 - E1879. ⟨10.1073/pnas.1400749111⟩. ⟨hal-01712228⟩
111 Consultations
4 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More