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Journal Articles Journal of Cell Biology Year : 2015

A dynamic formin-dependent deep F-actin network in axons


Although actin at neuronal growth cones is well-studied, much less is known about actin organization and dynamics along axon shafts and presynaptic boutons. Using probes that selectively label filamentous-actin (F-actin), we found focal "actin hotspots" along axons-spaced ∼3-4 µm apart-where actin undergoes continuous assembly/disassembly. These foci are a nidus for vigorous actin polymerization, generating long filaments spurting bidirectionally along axons-a phenomenon we call "actin trails." Super-resolution microscopy reveals intra-axonal deep actin filaments in addition to the subplasmalemmal "actin rings" described recently. F-actin hotspots colocalize with stationary axonal endosomes, and blocking vesicle transport diminishes the actin trails, suggesting mechanistic links between vesicles and F-actin kinetics. Actin trails are formin-but not Arp2/3-dependent and help enrich actin at presynaptic boutons. Finally, formin inhibition dramatically disrupts synaptic recycling. Collectively, available data suggest a two-tier F-actin organization in axons, with stable "actin rings" providing mechanical support to the plasma membrane and dynamic "actin trails" generating a flexible cytoskeletal network with putative physiological roles.


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hal-01701424 , version 1 (20-04-2018)



Archan Ganguly, Yong Tang, Lina Wang, Kelsey Ladt, Jonathan Loi, et al.. A dynamic formin-dependent deep F-actin network in axons. Journal of Cell Biology, 2015, 210 (3), pp.401 - 417. ⟨10.1083/jcb.201506110⟩. ⟨hal-01701424⟩
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