Polyproline as a Minimal Antifreeze Protein Mimic That Enhances the Cryopreservation of Cell Monolayers
Résumé
Tissue engineering,g ene therapy, drug screening, and emerging regenerative medicine therapies are fundamentally reliant on high-quality adherent cell culture,b ut current methods to cryopreserve cells in this format can give lowc ell yields and require large volumes of solvent " antifreezes ". Herein, we report polyproline as am inimum (bio)synthetic mimic of antifreezep roteins that is accessible by solution, solid-phase,a nd recombinant methods.W ed emonstrate that polyproline has ice recrystallisation inhibition activity linked to its amphipathic helix and that it enhances the DMSO cryopreservation of adherent cell lines.P olyproline mayb e av ersatile additive in the emerging field of macromolecular cryoprotectants.
Domaines
Matériaux
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Graham_et_al-2017-Angewandte_Chemie_International_Edition.pdf (1.96 Mo)
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