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Journal Articles Systematic and Applied Microbiology Year : 1988

Isolation of Microorganisms Producing 6-Deoxyhexose-Containing Polysaccharides


A screening of 27 collection strains and 135 strains isolated from carbohydrate rich waste materials permitted the selection of 13 strains which produced more 6-deoxyhexose-containing polysaccharides than a reference strain: Acinetobacter calcoaceticus BD 413 (ATCC 33304). The most productive strain identified as Klebsiella sp. excreted 1.6 g of rhamnose per liter of culture medium after improvement of certain culture conditions. The Klebsiella polysaccharide was recovered by precipitation which acetone and hydrolyzed. Rhamnose was separated by chromatography on Dowex 1 × 4 in borate buffer. Our study shows that sewage sludge is a good source of polysaccharide producers and that the use of hydrocarbon in selective medium and of a high carbon-nitrogen ratio promotes their development.
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hal-01685645 , version 1 (16-01-2018)



Marianne Graber, André Morin, Pierre Monsan. Isolation of Microorganisms Producing 6-Deoxyhexose-Containing Polysaccharides. Systematic and Applied Microbiology, 1988, 10 (2), pp.200-205. ⟨10.1016/S0723-2020(88)80038-9⟩. ⟨hal-01685645⟩


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