Detection of carbapenemase activity in Pseudomonas aeruginosa by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)

A new MALDI-TOF MS imipenem hydrolysis assay was developed to optimize detection of carbapenemase-producing strains of Pseudomonas aeruginosa. The method correctly discriminated carbapenemase producers (n=74) from non-producers (n=95) in 99.4% of cases, and successfully detected 100% GES-5 strains (n=5).

Mots clés

Carbapenemase Detection MALDI-TOF MS Pseudomonas aeruginosa

Domaines

Bactériologie

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https://hal.science/hal-01684649

Soumis le : lundi 15 janvier 2018-16:40:45

Dernière modification le : vendredi 31 mai 2024-14:53:05

Dates et versions

hal-01684649 , version 1 (15-01-2018)

Identifiants

HAL Id : hal-01684649 , version 1
DOI : 10.1016/j.mimet.2017.12.011
PUBMED : 29307737

Citer

Guillaume Miltgen, P. Plésiat, A. Mille, P. Chatelain, D. Fournier. Detection of carbapenemase activity in Pseudomonas aeruginosa by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS). Journal of Microbiological Methods, 2018, 145, pp.66 - 68. ⟨10.1016/j.mimet.2017.12.011⟩. ⟨hal-01684649⟩

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