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Article Dans Une Revue Molecular Pharmaceutics Année : 2018

Evaluation of the active targeting of melanin granules after intravenous injection of dendronized nanoparticles

Sébastien Piant
  • Fonction : Auteur
Christine Zbaraszczuk-Affolter
  • Fonction : Auteur
Sébastien Boutry
  • Fonction : Auteur
Elisa Cinotti
Sophie Laurent
Robert N. Muller
  • Fonction : Auteur
  • PersonId : 916596

Résumé

The biodistribution of dendronized iron oxides, NPs10@D1_DOTAGA and melanin-targeting NPs10@D1_ICF_DOTAGA, was studied in vivo using MRI and planar scintigraphy through [177Lu]Lu-radiolabeling. MRI experiments showed high contrast power of both dendronized nanoparticles (DPs) and hepatobiliary and urinary excretions. Little tumor uptake could be highlighted after intravenous injection probably as a consequence of the negatively charged DOTAGA-derivatized shell which reduces the diffusion across the cells' membrane. Planar scintigraphy images demonstrated a moderate specific tumor uptake of melanoma-targeted [177Lu]Lu-NPs10@D1_ICF_DOTAGA at 2 h post intravenous injection, and the highest tumor uptake of the control probe [177Lu]Lu-NPs10@D1_DOTAGA at 30min pi, probably due to the enhanced permeability and retention (EPR) effect. In addition, ex vivo Confocal microscopy (EVCM) studies showed a high specific targeting of human melanoma samples impregnated with NPs10@D1_ICF_Alexa647_ DOTAGA.
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Dates et versions

hal-01680955 , version 1 (11-01-2018)

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Citer

Catalina Bordeianu, Audrey Parat, Sébastien Piant, Aurélie Walter, Christine Zbaraszczuk-Affolter, et al.. Evaluation of the active targeting of melanin granules after intravenous injection of dendronized nanoparticles. Molecular Pharmaceutics, 2018, ⟨10.1021/acs.molpharmaceut.7b00904⟩. ⟨hal-01680955⟩
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