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Article Dans Une Revue Applied Microbiology and Biotechnology Année : 1994

Yeast viability related to water potential variation: influence of the transient phase

Résumé

The decrease rate of the water potential was found to have a great effect on yeasts submitted to hypertonic shifts. The application of slow and linear decreases of the water potential of the medium to cells of Saccharomyces cerevisiae demonstrated that the cells could survive (90 to 100% of viability) very low levels of water potential (=–101 MPa). Resistance of the cells was examined through viability measurements and cell volume changes. The kinetics of cell volume variation were measured continuously using an on-line image analysis system coupled to a microscope. No biological response of the cells occurred, due to the lack of a usable carbon-energy source in the medium. The viability level was found to be a function of the water exit flow rate from the cells. The denaturation of the membrane structure was assumed to be involved in such phenomena.

Dates et versions

hal-01668060 , version 1 (19-12-2017)

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Pierre-André Marechal, Patrick Gervais. Yeast viability related to water potential variation: influence of the transient phase. Applied Microbiology and Biotechnology, 1994, 42 (4), pp.617--622. ⟨10.1007/BF00173929⟩. ⟨hal-01668060⟩
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