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Article Dans Une Revue Methods in Molecular Biology Année : 2018

Isolation of Neural Stem and Progenitor Cells from the Adult Brain and Live Imaging of Their Cell Cycle with the FUCCI System.

Résumé

Neural stem cells (NSCs) enter quiescence in early embryonic stages to create a reservoir of dormant NSCs able to enter proliferation and produce neuronal precursors in the adult mammalian brain. Various approaches of fluorescent-activated cell sorting (FACS) have emerged to allow the distinction between quiescent NSCs (qNSCs), their activated counterpart (aNSCs), and the resulting progeny. In this article, we review two FACS techniques that can be used alternatively. We also show that their association with transgenic Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) mice allows an unprecedented overlook on the cell cycle dynamics of adult NSCs.
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Dates et versions

hal-01661733 , version 1 (12-12-2017)

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Alexandra Chicheportiche, Martial Ruat, François D Boussin, Mathieu Daynac. Isolation of Neural Stem and Progenitor Cells from the Adult Brain and Live Imaging of Their Cell Cycle with the FUCCI System.. Methods in Molecular Biology, 2018, Cellular Quiescence, 1686, pp.69-78. ⟨10.1007/978-1-4939-7371-2_5⟩. ⟨hal-01661733⟩
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