A species-specific DNA probe obtained from Streptococcus salivarius subsp. thermophilus detects strain restriction polymorphism - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue FEMS Microbiology Letters Année : 1991

A species-specific DNA probe obtained from Streptococcus salivarius subsp. thermophilus detects strain restriction polymorphism

Résumé

Genomic polymorphism in Streptococcus salivarius subsp. thermophilus was revealed by DNA restriction pattern analysis. A 4.2-kb variable DNA fragment was cloned from strain NST7 and hybridised with the DNA of 25 strains allowing an easy detection of intraspecific RFLP. Strong and weak hybridisation signals were observed and the latter were specifically revealed by a 2.1-kb fragment of the probe. Probe specificity was demonstrated by the absence of homology with DNA of strains belonging to 10 other species, with the exception of S. salivarius subsp. salivarius, confirming a close relationship between S. salivarius and S. thermophilus.
Fichier non déposé

Dates et versions

hal-01659159 , version 1 (08-12-2017)

Identifiants

  • HAL Id : hal-01659159 , version 1
  • PUBMED : 1679405

Citer

M Colmin, M Pebay, J M Simonet, B Decaris. A species-specific DNA probe obtained from Streptococcus salivarius subsp. thermophilus detects strain restriction polymorphism. FEMS Microbiology Letters, 1991, 65 (2), pp.123-8. ⟨hal-01659159⟩
41 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More