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Journal Articles Optica Year : 2017

High-speed polarization-resolved coherent Raman scattering imaging

Matthias Hofer
Naveen K. Balla
  • Function : Author
Sophie Brasselet

Abstract

Polarization-resolved coherent Raman scattering (polar-CRS) provides rich information on molecular orientational organization, with the strong advantages of being a label-free and chemically specific imaging method. Its implementation , however, strongly reduces the imaging acquisition rate, due to limits imposed by polarization tuning. Here we demonstrate fast-polar-CRS imaging based on combined electro-optic polarization and acousto-optic amplitude modulations , applicable to both stimulated Raman scattering and coherent anti-Stokes Raman scattering imaging. The proposed scheme adds polarization information without compromising the capacities of regular CRS intensity imaging ; increases the speed of orientational imaging by two orders of magnitude as compared with previous approaches; and does not require post-processing analyses. We show that this method permits sub-second timescale imaging of lipid order packing and local lipid membrane deformations in artificial lipid multilayers, but also in red blood cell ghosts, demonstrating its high sensitivity down to a single lipid bilayer membrane.
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Dates and versions

hal-01643484 , version 1 (13-04-2018)

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Matthias Hofer, Naveen K. Balla, Sophie Brasselet. High-speed polarization-resolved coherent Raman scattering imaging. Optica, 2017, 4 (7), ⟨10.1364/OPTICA.4.000795⟩. ⟨hal-01643484⟩
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