Characterisation and expression of phospholipases B from the opportunistic fungus Aspergillus fumigatus - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue FEMS Microbiology Letters Année : 2004

Characterisation and expression of phospholipases B from the opportunistic fungus Aspergillus fumigatus

Résumé

The phospholipase B family (PLB) are enzymes sharing phospholipase (PL), lysophospholipase (LPL) and lysophospholipase-transacylase (LPTA) activities. They have been shown to be important virulence factors in several human fungal pathogens including Candida albicans and Cryptococcus neoformans. Aspergillus fumigatus, a human opportunistic fungal pathogen leading to a high rate of mortality in immunosuppressed patients is known to possess an extracellular phospholipase B activity. In this paper, we report the molecular characterisation of three PLB genes from A. fumigatus (afplb) using degenerate primers in PCR amplification and data from the A. fumigatus genome project. They are expressed at 37 °C, and two of them (afplb1 and afplb3) are induced by lecithin. They encode proteins of 633, 588 and 630 amino acids, respectively, presenting together a T-Coffee score of 81. They also possess the amino acid triad responsible for enzymatic activity in the mammalian cytosolic PLA 2 and other fungal PLBs. AfPLB1 and afPLB3 are secreted with a cleaved signal peptide. The complete cDNA sequences were obtained by RACE-PCR for the two secreted afPLBs and probably account for the extracellular phospholipase activity previously reported in the culture media of A. fumigatus.
Fichier principal
Vignette du fichier
2004_FEMS_Shen_Brugere_revised-for Hal.pdf (180.06 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01612756 , version 1 (13-11-2020)

Identifiants

Citer

Da-Kang Shen, Ali Dehghan Noodeh, Abdolhassan Kazemi, Renée Grillot, Geoff Robson, et al.. Characterisation and expression of phospholipases B from the opportunistic fungus Aspergillus fumigatus. FEMS Microbiology Letters, 2004, 239 (1), pp.87 - 93. ⟨10.1016/j.femsle.2004.08.019⟩. ⟨hal-01612756⟩

Collections

UGA
16 Consultations
31 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More