Arabinoxylans and inulin modulate the luminal and mucosa-associated bacteria in vitro and in vivo - Archive ouverte HAL Accéder directement au contenu
Chapitre D'ouvrage Année : 2010

Arabinoxylans and inulin modulate the luminal and mucosa-associated bacteria in vitro and in vivo

Pieter van den Abbeele
  • Fonction : Auteur
Tom van de Wiele
Charlotte Grootaert
  • Fonction : Auteur
Willy Verstraete
  • Fonction : Auteur
Sam Possemiers
  • Fonction : Auteur correspondant

Résumé

There is increasing interest in gastro-intestinal microbiology to improve the balance between putative beneficial and harmful commensal microbiota. Mucosal microbes make closer contact with the host than luminal ones and could therefore more strongly affect host health. A short term in vitro adhesion assay was used to examine initial mucin colonisation by bacteria derived from the mixed microbial community of the ascending colon of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME). Further, long term prebiotic effects on the luminal and mucosa-associated microbial community (MAMC) were assessed in vitro and in vivo with respectively the SHIME and gnotobiotic rats. During the short term experiment, adhesion capacity differed substantially between bacteria and decreased from lactobacilli over faecal coliforms, bifidobacteria and clostridia to total anaerobes. In addition, intestinal water lowered adhesion compared to phosphate buffered saline. By processing the data to an Adhesion Related Prebiotic Index (AR-PI), it was found that intestinal water stimulated adherence of positive commensals. Arabinoxylans (AX) decreased the bacterial adhesion capacity (matrix independently), whereas inulin had less or no influence. During the long term experiment, group-specific DGGE analysis showed that although inoculated with the same human faecal microbiota, the in vivo and in vitro model both harbour distinct microbial communities. Both carbohydrates were shown to exert long term prebiotic effects on the luminal and mucosal microbial community. Interestingly, AX induced a shift towards a specific Bifidobacterium sp, both in the in vitro and in vivo model. The developed methodologies could be useful for the characterisation of the relevant but barely investigated mucosa-associated microbial community in health and disease (e.g. IBD) as well as for its modulation with functional foods like prebiotics.
Fichier non déposé

Dates et versions

hal-01608580 , version 1 (03-10-2017)

Identifiants

Citer

Pieter van den Abbeele, Tom van de Wiele, Charlotte Grootaert, Willy Verstraete, Philippe Gerard, et al.. Arabinoxylans and inulin modulate the luminal and mucosa-associated bacteria in vitro and in vivo. Dietary Fibre : New frontiers for food and health, Academic Publishers, 2010, 978-90-8686-692-2. ⟨10.3920/978-90-8686-692-2⟩. ⟨hal-01608580⟩
41 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More