Exfoliated epithelial cells : a source of bioligical information in preterm infants
Résumé
Context: Exfoliated epithelial cells can be isolated from several body fluids (i.e., breast milk,urines, and digestives fluids) as a cellular mixture (senescent, apoptotic, proliferative, or quiescentcells). By quantifying the degree of exfoliation, i.e. by assessing the loss of epithelial cells or oftheir specific molecular compounds, it is possible to evaluate pharmacological or nutritional stressin clinical trials as well as during epidemiological survey.Material & Methods: Written informed consents were obtained from both parents (or guardians) tocollect gastric fluid aspirates or stools from preterm infants. We have optimized a procedure toisolate epithelial cells from gastric fluid aspirates or stools of preterm infants and designed methodsto characterize exfoliated epithelial cells.Results: We have used two methods to measure relevant biological information from exfoliatedepithelial cells. The first is based on immunophenotyping by confocal imaging of gastric cellsisolated from gastric fluids aspirates (in order to evaluate the number of parietal cells, the possibilityto detect neuroendocrine cells or the occurrence of stem cell biomarkers). The second proposes torecover all eucaryote transcripts to perform qPCR analysis on cellular preparation recovered fromstools.Perspectives: We intend to design a system allowing the co-detection of messenger RNA andmicroRNA to evaluate the infant's physiological state.
Domaines
Alimentation et Nutrition
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