Collagen type I from bovine bone. Effect of animal age, bone anatomy and drying methodology on extraction yield, self-assembly, thermal behaviour and electrokinetic potential
Résumé
Natural
collagen
is
easily
available
from
animal
tissues
such
as
bones.
Main
limitations
reported
in
the
use
of
natural
collagen
are
heterogeneity
and
loss
of
integrity
during
recovery.
However,
its
natural
complexity,
functionality
and
bioactivity
still
remain
to
be
achieved
through
synthetic
and
recombinant
ways.
Variability
of
physicochemical
properties
of
collagen
extracted
from
bovine
bone
by
acetic
acid
was
then
investigated
taking
into
account
endogenous
and
exogenous
factors.
Endogenous:
bovine’s
bones
age
(4
and
7
years)
and
anatomy
(femur
and
tibia);
exogenous:
thermal
treatments
(spray-drying
and
lyophilisation).
Scanning
electron
microscopy,
spectroscopy
(EDS,
FTIR,
UV/Vis
and
CD),
differential
scan-
ning
calorimetry
(DSC),
centesimal
composition,
mass
spectrometry,
amino
acids
and
zeta-potential
analysis
were
used
for
the
purpose.
Age
correlated
negatively
with
yield
of
recovery
and
positively
with
minerals
and
proteoglycans
con-
tent.
Comparing
the
anatomy,
higher
yields
were
found
for
tibias,
and
higher
stability
of
tibias
collagen
in
solution
was
noticed.
Whatever
the
age
and
the
anatomy,
collagens
were
able
to
renature
and
to
self-
assemble
into
tri-dimensional
structures.
Nonetheless
thermal
stability
and
kinetics
of
renaturation
were
different.
Variability
of
natural
collagen
with
bone
age
and
anatomy,
and
drying
methodology,
may
be
a
crucial
advantage
to
conceive
tailor-made
applications
in
either
the
biological
or
technical
sector.