Functional expression of P-glycoprotein in an immortalised cell line of rat brain endothelial cells, RBE4 - Archive ouverte HAL
Article Dans Une Revue Journal of Neurochemistry Année : 1996

Functional expression of P-glycoprotein in an immortalised cell line of rat brain endothelial cells, RBE4

Résumé

The presence of P-glycoprotein in the cell plasma membrane limits the penetration of many cytotoxic substances into cells that express the gene product. There is considerable evidence also to indicate that P-glycoprotein is expressed as part of the normal blood-brain barrier in the luminal membranes of the cerebral capillary endothelial cells, where it presumably performs a protective function for the brain. This report describes the functional expression of P-glycoprotein in an immortalised cell line, RBE4, derived from rat cerebral capillary endothelial cells. The expression of P-glycoprotein is demonstrated by western immunoblotting and by immunogold and fluorescent staining with monoclonal antibodies. The cellular accumulation of [3H]colchicine and [3H]-vinblastine is investigated and shown to be enhanced by the presence of azidothymidine, chlorpromazine, verapamil, cyclosporin A, and PSC 833 ([3'-keto-Bmt1]-[Val2]-cyclosporin) at 50 or 100 microM concentration. It is concluded that the RBE4 cell line is a valuable tool for investigating the mechanisms of P-glycoprotein activity both in the blood-brain barrier and in multidrug resistance in general.
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Dates et versions

hal-01606068 , version 1 (02-10-2017)

Identifiants

  • HAL Id : hal-01606068 , version 1
  • PRODINRA : 406321
  • PUBMED : 8752104
  • WOS : A1996VD23200012

Citer

David J. Begley, Delphine Lechardeur, Zheng Duan Chen, Christopher Rollinson, Michèle Bardoul, et al.. Functional expression of P-glycoprotein in an immortalised cell line of rat brain endothelial cells, RBE4. Journal of Neurochemistry, 1996, 67 (3), pp.988-995. ⟨hal-01606068⟩

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