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Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2017

Structure of the Arabidopsis TOPLESS corepressor provides insight into the evolution of transcriptional repression

Résumé

Transcriptional repression involves a class of proteins called corepressors that link transcription factors to chromatin remodeling complexes. In plants such as Arabidopsis thaliana, the most prominent corepressor is TOPLESS (TPL), which plays a key role in hormone signaling and development. Here we present the crystallographic structure of the Arabidopsis TPL N-terminal region comprising the LisH and CTLH (C-terminal to LisH) domains and a newly identified third region, which corresponds to a CRA domain. Comparing the structure of TPL with the mammalian TBL1, which shares a similar domain structure and performs a parallel corepressor function, revealed that the plant TPLs have evolved a new tetramerization interface and unique and highly conserved surface for interaction with repressors. Using site-directed mutagenesis, we validated those surfaces in vitro and in vivo and showed that TPL tetramerization and repressor binding are interdependent. Our results illustrate how evolution used a common set of protein domains to create a diversity of corepressors, achieving similar properties with different molecular solutions.
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hal-01605698 , version 1 (25-05-2020)

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Raquel Martin-Arevalillo, Max H. Nanao, Antoine Larrieu, Thomas Vinos-Poyo, David Mast, et al.. Structure of the Arabidopsis TOPLESS corepressor provides insight into the evolution of transcriptional repression. Proceedings of the National Academy of Sciences of the United States of America, 2017, 114 (30), pp.8107-8112. ⟨10.1073/pnas.1703054114⟩. ⟨hal-01605698⟩
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