Genome sequence of the necrotrophic plant pathogen Pythium ultimum reveals original pathogenicity mechanisms and effector repertoire - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Genome Biology Année : 2010

Genome sequence of the necrotrophic plant pathogen Pythium ultimum reveals original pathogenicity mechanisms and effector repertoire

Liliana Cano
Sylvain Raffaele
Joe Win
Gordon W Beakes
  • Fonction : Auteur
Claire M M Gachon
Paul Morris
Vipaporn Phuntmart
  • Fonction : Auteur
Sophien Kamoun

Résumé

Background: Pythium ultimum is a ubiquitous oomycete plant pathogen responsible for a variety of diseases on a broad range of crop and ornamental species. Results: The P. ultimum genome (42.8 Mb) encodes 15,290 genes and has extensive sequence similarity and synteny with related Phytophthora species, including the potato blight pathogen Phytophthora infestans. Whole transcriptome sequencing revealed expression of 86% of genes, with detectable differential expression of suites of genes under abiotic stress and in the presence of a host. The predicted proteome includes a large repertoire of proteins involved in plant pathogen interactions, although, surprisingly, the P. ultimum genome does not encode any classical RXLR effectors and relatively few Crinkler genes in comparison to related phytopathogenic oomycetes. A lower number of enzymes involved in carbohydrate metabolism were present compared to Phytophthora species, with the notable absence of cutinases, suggesting a significant difference in virulence mechanisms between P. ultimum and more host-specific oomycete species. Although we observed a high degree of orthology with Phytophthora genomes, there were novel features of the P. ultimum proteome, including an expansion of genes involved in proteolysis and genes unique to Pythium. We identified a small gene family of cadherins, proteins involved in cell adhesion, the first report of these in a genome outside the metazoans. Conclusions: Access to the P. ultimum genome has revealed not only core pathogenic mechanisms within the oomycetes but also lineage-specific genes associated with the alternative virulence and lifestyles found within the pythiaceous lineages compared to the Peronosporaceae.

Mots clés

Fichier principal
Vignette du fichier
document.pdf (2.83 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

hal-01605006 , version 1 (14-05-2020)

Licence

Paternité

Identifiants

Citer

C André Lévesque, Henk Brouwer, Liliana Cano, John P Hamilton, Carson Holt, et al.. Genome sequence of the necrotrophic plant pathogen Pythium ultimum reveals original pathogenicity mechanisms and effector repertoire. Genome Biology, 2010, 11 (7), ⟨10.1186/gb-2010-11-7-r73⟩. ⟨hal-01605006⟩
99 Consultations
22 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More