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Article Dans Une Revue Biophysical Journal Année : 2015

Single cell wall nonlinear mechanics revealed by a multiscale analysis of AFM force-indentation curves

Résumé

Individual plant cells are rather complex mechanical objects. Despite the fact that their wall mechanical strength may be weakened by comparison with their original tissue template, they nevertheless retain some generic properties of the mother tissue, namely the viscoelasticity and the shape of their walls, which are driven by their internal hydrostatic turgor pressure. This viscoelastic behavior, which affects the power- law response of these cells when indented by an atomic force cantilever with a pyramidal tip, is also very sensitive to the culture media. To our knowledge, we develop here an original analyzing method, based on a multiscale decomposition of force-indentation curves, that reveals and quantifies for the first time the nonlinearity of the mechanical response of living single plant cells upon mechanical deformation. Further comparing the nonlinear strain responses of these isolated cells in three different media, we reveal an alteration of their linear bending elastic regime in both hyper- and hypotonic conditions.

Dates et versions

hal-01601462 , version 1 (02-10-2017)

Identifiants

Citer

Simona Digiuni, Annik Berne-Dedieu, Cristina Martinez-Torres, Judit Szecsi, Mohammed Bendahmane, et al.. Single cell wall nonlinear mechanics revealed by a multiscale analysis of AFM force-indentation curves. Biophysical Journal, 2015, 108 (9), pp.2235-2248. ⟨10.1016/j.bpj.2015.02.024⟩. ⟨hal-01601462⟩
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