Transcriptome analysis of the Populus trichocarpa–Rhizophagus irregularis Mycorrhizal Symbiosis - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Plant and Cell Physiology Année : 2017

Transcriptome analysis of the Populus trichocarpa–Rhizophagus irregularis Mycorrhizal Symbiosis

Résumé

Nutrient transfer is a key feature of the arbuscular mycorrhizal (AM) symbiosis. Valuable mineral nutrients are transferred from the AM fungus to the plant, increasing its fitness and productivity, and, in exchange, the AM fungus receives carbohydrates as an energy source from the plant. Here, we analyzed the transcriptome of the Populus trichocarpa-Rhizophagus irregularis symbiosis using RNA-sequencing of non-mycorrhizal or mycorrhizal fine roots, with a focus on the effect of nitrogen (N) starvation. In R. irregularis, we identified 1,015 differentially expressed genes, whereby N starvation led to a general induction of gene expression. Genes of the functional classes of cell growth, membrane biogenesis and cell structural components were highly abundant. Interestingly, N starvation also led to a general induction of fungal transporters, indicating increased nutrient demand upon N starvation. In non-mycorrhizal P. trichocarpa roots, 1,341 genes were differentially expressed under N starvation. Among the 953 down-regulated genes in N starvation, most were involved in metabolic processes including amino acids, carbohydrate and inorganic ion transport, while the 342 up-regulated genes included many defense-related genes. Mycorrhization led to the up-regulation of 549 genes mainly involved in secondary metabolite biosynthesis and transport; only 24 genes were down-regulated. Mycorrhization specifically induced expression of three ammonium transporters and one phosphate transporter, independently of the N conditions, corroborating the hypothesis that these transporters are important for symbiotic nutrient exchange. In conclusion, our data establish a framework of gene expression in the two symbiotic partners under high-N and low-N conditions.

Dates et versions

hal-01598827 , version 1 (29-09-2017)

Identifiants

Citer

Silvia Calabrese, Annegret Kohler, Annette Niehl, Claire Veneault-Fourrey, Thomas Boller, et al.. Transcriptome analysis of the Populus trichocarpa–Rhizophagus irregularis Mycorrhizal Symbiosis: Regulation of Plant and Fungal Transportomes under Nitrogen Starvation. Plant and Cell Physiology, 2017, 58 (6), pp.1003-1017. ⟨10.1093/pcp/pcx044⟩. ⟨hal-01598827⟩
102 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More