Honeybee locomotion is impaired by Am-CaV3 low voltage-activated Ca(2+) channel antagonist. - Archive ouverte HAL Access content directly
Journal Articles Scientific Reports Year : 2017

Honeybee locomotion is impaired by Am-CaV3 low voltage-activated Ca(2+) channel antagonist.

Claude Collet
Mercedes Charreton
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Abstract

Voltage-gated Ca(2+) channels are key transducers of cellular excitability and participate in several crucial physiological responses. In vertebrates, 10 Ca(2+) channel genes, grouped in 3 families (CaV1, CaV2 and CaV3), have been described and characterized. Insects possess only one member of each family. These genes have been isolated in a limited number of species and very few have been characterized although, in addition to their crucial role, they may represent a collateral target for neurotoxic insecticides. We have isolated the 3 genes coding for the 3 Ca(2+) channels expressed in Apis mellifera. This work provides the first detailed characterization of the honeybee T-type CaV3 Ca(2+) channel and demonstrates the low toxicity of inhibiting this channel. Comparing Ca(2+) currents recorded in bee neurons and myocytes with Ca(2+) currents recorded in Xenopus oocytes expressing the honeybee CaV3 gene suggests native expression in bee muscle cells only. High-voltage activated Ca(2+) channels could be recorded in the somata of different cultured bee neurons. These functional data were confirmed by in situ hybridization, immunolocalization and in vivo analysis of the effects of a CaV3 inhibitor. The biophysical and pharmacological characterization and the tissue distribution of CaV3 suggest a role in honeybee muscle function.
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hal-01595255 , version 1 (26-09-2017)

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Matthieu Rousset, Claude Collet, Thierry Cens, Florian Bastin, Valérie Raymond, et al.. Honeybee locomotion is impaired by Am-CaV3 low voltage-activated Ca(2+) channel antagonist.. Scientific Reports, 2017, 7, pp.41782. ⟨10.1038/srep41782⟩. ⟨hal-01595255⟩
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