Toward universal forward genetics: using a draft genome sequence of the nematode oscheius tipulae to identify mutations affecting vulva development - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Genetics Année : 2017

Toward universal forward genetics: using a draft genome sequence of the nematode oscheius tipulae to identify mutations affecting vulva development

Résumé

Mapping-by-sequencing has become a standard method to map and identify phenotype-causing mutations in model species. Here, we show that a fragmented draft assembly is sufficient to perform mapping-by-sequencing in nonmodel species. We generated a draft assembly and annotation of the genome of the free-living nematode Oscheius tipulae, a distant relative of the model Caenorhabditis elegans We used this draft to identify the likely causative mutations at the O. tipulae cov-3 locus, which affect vulval development. The cov-3 locus encodes the O. tipulae ortholog of C. elegans mig-13, and we further show that Cel-mig-13 mutants also have an unsuspected vulval-development phenotype. In a virtuous circle, we were able to use the linkage information collected during mutant mapping to improve the genome assembly. These results showcase the promise of genome-enabled forward genetics in nonmodel species.
Fichier principal
Vignette du fichier
1747.pdf (1.59 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-01595198 , version 1 (23-01-2024)

Identifiants

Citer

Fabrice Besnard, Georgios Koutsovoulos, Sana Dieudonne, Mark Blaxter, Marie-Anne Félix. Toward universal forward genetics: using a draft genome sequence of the nematode oscheius tipulae to identify mutations affecting vulva development. Genetics, 2017, 206 (4), pp.1747-1761. ⟨10.1534/genetics.117.203521⟩. ⟨hal-01595198⟩
74 Consultations
4 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More