Matrix-Free DNP-Enhanced NMR Spectroscopy of Liposomes Using a Lipid-Anchored Biradical - Archive ouverte HAL
Journal Articles Chemistry - A European Journal Year : 2015

Matrix-Free DNP-Enhanced NMR Spectroscopy of Liposomes Using a Lipid-Anchored Biradical

Hiroki Takahashi
Vincent Maurel
Daniel Lee
Sabine Hediger
Gaël de Paëpe

Abstract

Magic-angle spinning dynamic nuclear polarization (MAS-DNP) has been proven to be a powerful technique to enhance the sensitivity of solid-state NMR (SSNMR) in a wide range of systems. Here, we show that DNP can be used to polarize lipids using a lipid-anchored polarizing agent. More specifically, we introduce a C16-functionalized biradical, which allows localization of the polarizing agents in the lipid bilayer and DNP experiments to be performed in the absence of excess cryo-protectant molecules (glycerol, dimethyl sulfoxide, etc.). This constitutes another original example of the matrix-free DNP approach that we recently introduced.

Dates and versions

hal-01592879 , version 1 (25-09-2017)

Identifiers

Cite

Carlos Fernandez-De-Alba, Hiroki Takahashi, Alexandre Richard, Yves Chenavier, Lionel Dubois, et al.. Matrix-Free DNP-Enhanced NMR Spectroscopy of Liposomes Using a Lipid-Anchored Biradical. Chemistry - A European Journal, 2015, 21 (12), pp.4512-4517. ⟨10.1002/chem.201404588⟩. ⟨hal-01592879⟩
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