Prototype of a Wireless System for Electrical Impedance Spectroscopy: Demonstration of Real Time Measurements on Cells and Biological Medium
Résumé
A wireless and portable system, consisting in an
electronic board and a computer software graphical interface,
is presented as a feasible way to do electrochemical impedance
spectroscopy of cells and biological medium. It is designed to
work inside a culture chamber performing impedance
measurement in the frequency range of 64Hz to 200KHz. The
board is equipped with a Bluetooth Low Energy (BLE) module
allowing it to be wirelessly controlled. The software interface
(also called ISMI) is coded with all required functionalities to
manage the parameters of the board such as start frequency
and sweep, measuring intervals, data acquisition and
visualization and also a function to perform automatic
measuring between desired time intervals during whole day for
many days. A validation of the system shows an average error
of about 1.9% and a measuring test of human fibroblast cells
shows a good performance of the system. The proposed system
is an introductory step in the study of cells related to fibrous
tissue induced by implants showing to be a viable and
reproducibility-improving approach for such analysis.