Metagenomic-based impact study of transgenic grapevine rootstock on its associated virome and soil bacteriome - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Plant Biotechnology Journal Année : 2017

Metagenomic-based impact study of transgenic grapevine rootstock on its associated virome and soil bacteriome

Jean-Michel Hily
  • Fonction : Auteur
  • PersonId : 1204339
Monique Beuve
  • Fonction : Auteur
  • PersonId : 1179501
Gérard Demangeat
  • Fonction : Auteur
  • PersonId : 1204341
Armelle Marais
Thierry T. Candresse
Pascal Simonet
Olivier Lemaire
  • Fonction : Auteur
  • PersonId : 1204343

Résumé

For some crops, the only possible approach to gain a specific trait requires genome modification. The development of virus-resistant transgenic plants based on the pathogen-derived resistance strategy has been a success story for over three decades. However, potential risks associated with the technology, such as horizontal gene transfer (HGT) of any part of the transgene to an existing gene pool, have been raised. Here, we report no evidence of any undesirable impacts of genetically modified (GM) grapevine rootstock on its biotic environment. Using state of the art metagenomics, we analysed two compartments in depth, the targeted Grapevine fanleaf virus (GFLV) populations and nontargeted root-associated microbiota. Our results reveal no statistically significant differences in the genetic diversity of bacteria that can be linked to the GM trait. In addition, no novel virus or bacteria recombinants of biosafety concern can be associated with transgenic grapevine rootstocks cultivated in commercial vineyard soil under greenhouse conditions for over 6 years.
Fichier principal
Vignette du fichier
Publis17-svqv-hily_1.pdf (1.38 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01588179 , version 1 (26-05-2020)

Licence

Paternité - Partage selon les Conditions Initiales

Identifiants

Citer

Jean-Michel Hily, Sandrine Demanèche, Nils Poulicard, Mélanie Tannières, Samia Djennane, et al.. Metagenomic-based impact study of transgenic grapevine rootstock on its associated virome and soil bacteriome. Plant Biotechnology Journal, 2017, 16 (1), pp.208-220. ⟨10.1111/pbi.12761⟩. ⟨hal-01588179⟩
301 Consultations
43 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More