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Article Dans Une Revue Bioconjugate Chemistry Année : 2007

Lipophosphoramidates as Lipidic Part of Lipospermines for Gene Delivery

Résumé

The DNA compacting properties of polyamines (especially spermine) are well-known, hence the use of spermine as the cationic part in several synthetic DNA carriers. Here, we describe the synthesis of modified spermines, with a "lipophosphoramidate" as the lipidic part, and their use for efficient in vitro transfection. Physicochemical measurements (particle size, zeta potentials, pKa determination) and gel retardation assays were also performed. Theoretical membrane-disrupting ability was established by FRET. Taken together, our results indicate that lipophosphoramidates constitute an interesting alternative to "classical" lipidic parts of cationic lipids used as DNA carriers.
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Dates et versions

hal-01581383 , version 1 (30-09-2022)

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Paternité - Pas d'utilisation commerciale

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François Lamarche, Mathieu Mével, Tristan Montier, Laure Burel-Deschamps, Philippe Giamarchi, et al.. Lipophosphoramidates as Lipidic Part of Lipospermines for Gene Delivery. Bioconjugate Chemistry, 2007, 18 (5), pp.1575-1582. ⟨10.1021/bc070070w⟩. ⟨hal-01581383⟩
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